Dengue NS1 Recombinant Protein

Product code: PVBSP801                                                               
For laboratory use

Shipping: Shipped on Dry Ice
Storage Conditions: Store at -20 °C, avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, FPLC)

Source: E.Coli
Form: Liquid (Supplied in 50 mM Tris pH 8.5)
Molecular Weight: 40 kDa

Applications:

• Lateral Flow Immunoassay: Used for the detection of Dengue NS1 antibodies in patient samples, aiding in the early diagnosis of Dengue fever.
• ELISA: Ideal for quantitative measurement of Dengue NS1 antibodies, crucial for diagnosing and monitoring Dengue infection.
• Western Blots: Confirms the presence of Dengue NS1 antibodies in infected samples.

Description:
The Dengue NS1 recombinant protein includes the non-structural protein 1 (NS1) from Dengue virus. This protein is essential for the early diagnosis of Dengue fever, as it is one of the first markers to appear during infection. It is expressed in a bacterial system and purified using advanced chromatographic techniques to ensure high purity and activity.

Background:
Dengue virus is transmitted by mosquitoes and causes Dengue fever, which can lead to severe symptoms and complications. The NS1 protein is secreted during the early stages of infection and is a valuable marker for diagnosing Dengue fever. Early detection of NS1 is critical for timely treatment and management of the disease.

Specificity: Immunoreactive with samples from individuals infected with Dengue virus, ensuring accurate detection of Dengue NS1 antigen.

Anti-Dengue NS1 Monoclonal Antibody (Primary Antibody)

Product code: PVBSP802                                                                      
For laboratory use

Shipping: Shipped on Dry Ice
Storage Conditions: Store at -20 °C, avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 98 % (SDS-PAGE, FPLC)

Source: Mouse
Form: Liquid (Supplied in PBS, pH 7.4)
Molecular Weight: Approximately 150 kDa (IgG antibody)

Applications:

• Lateral Flow Immunoassay: Used for the detection of Dengue NS1 antigen present in patient samples .
• ELISA: Detects Dengue NS1 antigen, facilitating early diagnosis and monitoring of Dengue fever.
• Western Blots: Confirms the presence of Dengue NS1 antigen in patient samples.
• Immunohistochemistry: Allows for the visualization of Dengue NS1 antigen in tissue samples, aiding in research and diagnostic studies.
• Flow Cytometry: Utilized for identifying and quantifying Dengue NS1 antigen in various cell populations, critical for research on Dengue virus.

Description:
This primary monoclonal antibody is highly specific to Dengue NS1 protein. Produced using hybridoma technology, it is purified through proprietary chromatographic techniques to ensure high specificity and binding affinity. Provided in liquid form, it is supplied in PBS buffer to maintain stability and functionality.

Background:
Monoclonal antibodies against Dengue NS1 are essential for diagnosing Dengue fever and conducting research on the virus. They enable the detection of NS1, which is crucial for early diagnosis and monitoring of infection. This antibody plays a critical role in developing sensitive and specific diagnostic assays.

Specificity: Highly specific to Dengue NS1 antigen, ensuring precise detection in various applications.

Anti-Dengue NS1 Monoclonal Antibody (Secondary Antibody)

Product code: PVBSP803
For laboratory use

Shipping: Shipped on Dry Ice
Storage Conditions: Store at -20 °C, avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 98 % (SDS-PAGE, FPLC)

Source: Mouse
Form: Liquid (Supplied in PBS, pH 7.4)
Molecular Weight: Approximately 150 kDa (IgG antibody)

Applications:

• Lateral Flow Immunoassay: Used for the detection of Dengue NS1 antigen present in patient samples .
• ELISA: Utilized in a double antibody sandwich (DAS) format to detect and quantify Dengue NS1 antigen. In this method, the primary antibody captures the antigen, and this secondary antibody binds to a different epitope on the Dengue NS1 antigen, enhancing sensitivity and specificity.

• Western Blots: Facilitates the confirmation of Dengue NS1 antigen presence by binding to a different epitope of the antigen already captured by the primary antibody on the membrane.
• Immunohistochemistry: Enhances visualization of Dengue NS1 antigen in tissue samples by binding to a distinct epitope on the antigen, complementing primary antibody detection.
• Flow Cytometry: Applied to detect and quantify Dengue NS1 antigen in cell populations by binding to a different epitope on the antigen than the primary antibody, improving analysis accuracy.

Description:
This secondary monoclonal antibody is designed to bind specifically to a different epitope on the Dengue NS1 antigen than the primary antibody. Produced using hybridoma technology and purified through proprietary chromatographic techniques, it ensures high specificity and binding affinity. Supplied in liquid form with PBS buffer, it is optimized for use in the double antibody sandwich (DAS) assay formats. In DAS assays, the secondary antibody binds to a distinct epitope on the antigen that is already captured by the primary antibody, allowing for enhanced detection and quantification.

Background:
The double antibody sandwich (DAS) principle involves capturing the antigen between two antibodies: a primary antibody that binds to one epitope on the antigen and a secondary antibody that binds to a different epitope on the same antigen. This approach improves sensitivity and specificity by amplifying the signal and reducing background noise. The secondary antibody, conjugated with a detectable label, allows for precise and reliable measurement of the antigen of interest.

Specificity: Highly specific to a different epitope on Dengue NS1 antigen than the primary antibody, ensuring accurate and enhanced detection in double antibody sandwich assays.